Journal: Radiation research

Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay

doi: 10.1667/RADE-22-00206.1

Figure Lengend Snippet: Overview of Participating Teams, Utilized Platforms, Number and Names of Genes or Gene Combinations Used, the Origin of Calibration Samples, and Further Details

Article Snippet: TaqMan assays SYBR Green assay , FDXR (Hs00244586_ml), GDF15 (Hs00171132_ml) , BAX (Hs00180269_ml), BBC3 (Hs00248075_ml), CDKN1A (Hs00355782_ml), DDB2 (Hs03044953_ml), FDXR (Hs00244586_ml), GADD45A (Hs00169255_ml), GDF15 (Hs00171132_ml), TNFSF4 (Hs00182411_ml) , CDKN1A-F: AGACCAGCATGACAGATTTCTACC; CDKN1A-R: CTTCCTGTGGGCGGATTAGG; DDB2-F: AGCATCACTGGGCTGAAGTT; DDB2-R: TGGTGTCTGAGCTGGCAAAA; FDX-F: TGGAGAGAACGGACATCACG; FDX-R: AGCCACACTGTCTTCACTCG , GADD45a for: ACTGCGTGCTGGTGACGAAT, GADD45a rev: GTTGACTTAAGGCAGGATCCTTCCA; FDXR for: TGGATGTGCCAGGCCTCTAC, FDXR rev: TGAGGAAGCTGTCAGTCATGGTT; CDKN1A for: CCTGGAGACTCTCAGGGTCGAAA, CDKN1A rev: GCGTTTGGAGTGGTAGAAATCTGTCA; MDM2 for: TATCAGGCAGGGGAGAGTGATACA, MDM2 rev: CCAACATCTGTTGCAATGTGATGGAA; 18S for: GCTTAATTTGACTCAACACGGGA, 18S rev: AGCTATCAATCTGTCAATCCTGTCC. , TaqMan ® assay: DDB2 (Hs00172068_ml), FDXR (HS01031617_ml), ITFG1: Hs01061271_ml SYBR Green assay: CDKN1A F:CCT CAT CCC GTG TTC TCC TTT CDKN1A R: GTA CCA CCC AGC GGA CAA GT GAPDH F: CGA CCA CTT TGT CAA GCT CA GAPDH R: AGG GGT CTA CAT GGC AAC TG HPRT F: TGA CAC TGG CAA AAC AAT GCA HPRT R: GGT CCT TTT CAC CAG CAA GCT , FDXR (HS01031617_ml) , DDB2 (Hs00172068_ml), FDXR (HS01031617_ml) , RNA amount used for cDNA synthesis , 0.2 μg; 1.65 μg per array.

Techniques: Generated

Journal: Radiation research

Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay

doi: 10.1667/RADE-22-00206.1

Figure Lengend Snippet: Overview of Methodological Details of Either qRT-PCR (Quantitative Reverse Transcription Polymerase Chain Reaction) or Microarrays Used by the Contributing Teams

Article Snippet: TaqMan assays SYBR Green assay , FDXR (Hs00244586_ml), GDF15 (Hs00171132_ml) , BAX (Hs00180269_ml), BBC3 (Hs00248075_ml), CDKN1A (Hs00355782_ml), DDB2 (Hs03044953_ml), FDXR (Hs00244586_ml), GADD45A (Hs00169255_ml), GDF15 (Hs00171132_ml), TNFSF4 (Hs00182411_ml) , CDKN1A-F: AGACCAGCATGACAGATTTCTACC; CDKN1A-R: CTTCCTGTGGGCGGATTAGG; DDB2-F: AGCATCACTGGGCTGAAGTT; DDB2-R: TGGTGTCTGAGCTGGCAAAA; FDX-F: TGGAGAGAACGGACATCACG; FDX-R: AGCCACACTGTCTTCACTCG , GADD45a for: ACTGCGTGCTGGTGACGAAT, GADD45a rev: GTTGACTTAAGGCAGGATCCTTCCA; FDXR for: TGGATGTGCCAGGCCTCTAC, FDXR rev: TGAGGAAGCTGTCAGTCATGGTT; CDKN1A for: CCTGGAGACTCTCAGGGTCGAAA, CDKN1A rev: GCGTTTGGAGTGGTAGAAATCTGTCA; MDM2 for: TATCAGGCAGGGGAGAGTGATACA, MDM2 rev: CCAACATCTGTTGCAATGTGATGGAA; 18S for: GCTTAATTTGACTCAACACGGGA, 18S rev: AGCTATCAATCTGTCAATCCTGTCC. , TaqMan ® assay: DDB2 (Hs00172068_ml), FDXR (HS01031617_ml), ITFG1: Hs01061271_ml SYBR Green assay: CDKN1A F:CCT CAT CCC GTG TTC TCC TTT CDKN1A R: GTA CCA CCC AGC GGA CAA GT GAPDH F: CGA CCA CTT TGT CAA GCT CA GAPDH R: AGG GGT CTA CAT GGC AAC TG HPRT F: TGA CAC TGG CAA AAC AAT GCA HPRT R: GGT CCT TTT CAC CAG CAA GCT , FDXR (HS01031617_ml) , DDB2 (Hs00172068_ml), FDXR (HS01031617_ml) , RNA amount used for cDNA synthesis , 0.2 μg; 1.65 μg per array.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Microarray, Isolation, Red Blood Cell Lysis, Control, Concentration Assay, Sequencing, cDNA Synthesis, Labeling, SYBR Green Assay, Multiplex Assay, TaqMan Assay, Real-time Polymerase Chain Reaction, Software, Extraction

Journal: Radiation research

Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay

doi: 10.1667/RADE-22-00206.1

Figure Lengend Snippet: The Table Depicts Team Contributions (from Left to Right) Regarding Employed Genes, Reported Dose Estimates per Reference Sample 1–3, Differences among Reported and Reference Dose-Values as well as the Summed Absolute Difference over all Reference Samples (SAD), a Correct (Yes) or Incorrect (No) Order of Dose Estimates (from Lowest to Highest) Corresponding to Three Dose Categories [Unexposed, Low (1.2 Gy) and Highly Exposed (3.5 Gy)], the Use of FDXR Gene Expression Changes for dose estimation, as well as the Report Time

Article Snippet: TaqMan assays SYBR Green assay , FDXR (Hs00244586_ml), GDF15 (Hs00171132_ml) , BAX (Hs00180269_ml), BBC3 (Hs00248075_ml), CDKN1A (Hs00355782_ml), DDB2 (Hs03044953_ml), FDXR (Hs00244586_ml), GADD45A (Hs00169255_ml), GDF15 (Hs00171132_ml), TNFSF4 (Hs00182411_ml) , CDKN1A-F: AGACCAGCATGACAGATTTCTACC; CDKN1A-R: CTTCCTGTGGGCGGATTAGG; DDB2-F: AGCATCACTGGGCTGAAGTT; DDB2-R: TGGTGTCTGAGCTGGCAAAA; FDX-F: TGGAGAGAACGGACATCACG; FDX-R: AGCCACACTGTCTTCACTCG , GADD45a for: ACTGCGTGCTGGTGACGAAT, GADD45a rev: GTTGACTTAAGGCAGGATCCTTCCA; FDXR for: TGGATGTGCCAGGCCTCTAC, FDXR rev: TGAGGAAGCTGTCAGTCATGGTT; CDKN1A for: CCTGGAGACTCTCAGGGTCGAAA, CDKN1A rev: GCGTTTGGAGTGGTAGAAATCTGTCA; MDM2 for: TATCAGGCAGGGGAGAGTGATACA, MDM2 rev: CCAACATCTGTTGCAATGTGATGGAA; 18S for: GCTTAATTTGACTCAACACGGGA, 18S rev: AGCTATCAATCTGTCAATCCTGTCC. , TaqMan ® assay: DDB2 (Hs00172068_ml), FDXR (HS01031617_ml), ITFG1: Hs01061271_ml SYBR Green assay: CDKN1A F:CCT CAT CCC GTG TTC TCC TTT CDKN1A R: GTA CCA CCC AGC GGA CAA GT GAPDH F: CGA CCA CTT TGT CAA GCT CA GAPDH R: AGG GGT CTA CAT GGC AAC TG HPRT F: TGA CAC TGG CAA AAC AAT GCA HPRT R: GGT CCT TTT CAC CAG CAA GCT , FDXR (HS01031617_ml) , DDB2 (Hs00172068_ml), FDXR (HS01031617_ml) , RNA amount used for cDNA synthesis , 0.2 μg; 1.65 μg per array.

Techniques: Gene Expression

Journal: Chemsuschem

Article Title: Improving Plasma‐Catalytic Ammonia Synthesis Using a Coaxial Double‐Helix‐Electrode Reactor

doi: 10.1002/cssc.202502695

Figure Lengend Snippet: Models of three discharge configurations for the simulation study. (a) Internal single‐helix structure with winding pitches of 1, 3, and 5 mm; (b) double‐helix structure with winding pitches of 1, 3, and 5 mm; and (c) conventional coaxial DBD simulation structure schematic.

Article Snippet: Thus, depending on the winding pitch, the double‐helix configuration produces 127%, 96%, and 66% higher R values than the conventional system.

Techniques:

Journal: Chemsuschem

Article Title: Improving Plasma‐Catalytic Ammonia Synthesis Using a Coaxial Double‐Helix‐Electrode Reactor

doi: 10.1002/cssc.202502695

Figure Lengend Snippet: Comparison of plasma‐only ammonia synthesis performance under different electrode materials and winding spacings in the double‐helix DBD reactor. (a–c) NH 3 concentration as a function of SEI for Cu, Ni, and W electrodes, respectively, at winding spacings of 1, 3, and 5 mm. The performance of the conventional coaxial DBD reactor is included for comparison. (d) Influence of electrode material at a fixed winding pitch of 1 mm. (Experiment conditions: total gas flow rate = 40 mL min −1 ; H 2 /N 2 = 1:1 (v/v); atmospheric pressure; sine waveform input; discharge frequency = 7 kHz; applied voltage = 6–10 kV).

Article Snippet: Thus, depending on the winding pitch, the double‐helix configuration produces 127%, 96%, and 66% higher R values than the conventional system.

Techniques: Comparison, Clinical Proteomics, Concentration Assay

Journal: Chemsuschem

Article Title: Improving Plasma‐Catalytic Ammonia Synthesis Using a Coaxial Double‐Helix‐Electrode Reactor

doi: 10.1002/cssc.202502695

Figure Lengend Snippet: Ammonia synthesis performance of the double‐helix DBD reactor with and without catalyst incorporation: (a) NH 3 concentration as a function of SEI at different input powers; (b) corresponding energy yield ( E NH3 ) under the same conditions of (a); (c) NH 3 concentration as a function of SEI at different gas flow rates under fixed discharge power (20 W); (d) corresponding E NH3 for the conditions shown in (c). Results from the plasma‐only and conventional coaxial DBD reactors are included for comparison. (Experiment conditions: total gas flow rate = 40 mL min −1 ; H 2 /N 2 = 1:1 (v/v); atmospheric pressure; sine waveform input; discharge frequency = 7 kHz; applied voltage = 6–10 kV).

Article Snippet: Thus, depending on the winding pitch, the double‐helix configuration produces 127%, 96%, and 66% higher R values than the conventional system.

Techniques: Concentration Assay, Clinical Proteomics, Comparison

Journal: Chemsuschem

Article Title: Improving Plasma‐Catalytic Ammonia Synthesis Using a Coaxial Double‐Helix‐Electrode Reactor

doi: 10.1002/cssc.202502695

Figure Lengend Snippet: (a) Long‐term ammonia synthesis performance of the double‐helix DBD reactor coupled with the Ni/Al 2 O 3 catalyst under pulsed power excitation, showing stable energy efficiency and ammonia synthesis rate over 50 h of continuous operation (experiment conditions: total gas flow rate = 150 mL min −1 with H 2 /N 2 = 1:1 (v/v); atmospheric pressure; pulsed waveform input; discharge frequency: 12 kHz; applied voltage: 10 kV). (b) Benchmark comparison of ammonia synthesis rate and energy efficiency with representative plasma‐assisted systems reported in the literature.

Article Snippet: Thus, depending on the winding pitch, the double‐helix configuration produces 127%, 96%, and 66% higher R values than the conventional system.

Techniques: Comparison, Clinical Proteomics

Journal: Chemsuschem

Article Title: Improving Plasma‐Catalytic Ammonia Synthesis Using a Coaxial Double‐Helix‐Electrode Reactor

doi: 10.1002/cssc.202502695

Figure Lengend Snippet: Electric‐field simulation of different DBD reactor architectures. (a–c) Schematic diagrams of the three electrode configurations: (a) double‐helix structure, (b) internal single‐helix structure, and (c) conventional coaxial DBD. (d–f) Simulated electric‐field intensity distributions (cross‐sectional contours) corresponding to the structures shown in (a–c). (g) Radial electric‐field intensity profiles (electric field line 1) for the three structures at an applied voltage of 2 kV and a winding pitch of 1 mm. (h) Axial electric‐field intensity profiles (electric field line 2) under the same conditions, illustrating the periodic field variation along the electrode winding direction.

Article Snippet: Thus, depending on the winding pitch, the double‐helix configuration produces 127%, 96%, and 66% higher R values than the conventional system.

Techniques:

Journal: Chemsuschem

Article Title: Improving Plasma‐Catalytic Ammonia Synthesis Using a Coaxial Double‐Helix‐Electrode Reactor

doi: 10.1002/cssc.202502695

Figure Lengend Snippet: (a) High‐speed camera imaging of the instantaneous discharge evolution in the double‐helix, internal single‐helix, and conventional coaxial DBD reactors at different exposure times (0.5, 4, 8, 12, and 16 ms, under sine waveform input), illustrating differences in discharge initiation, propagation, and spatial uniformity. (b) Comparison of NH 3 concentration (bars) and total discharge event counts per cycle (blue markers) for Ni, Cu, and W electrodes at different winding pitches (1, 3, and 5 mm). Discharge events were obtained from current signal statistics, where forward discharges were defined as ≥+44 mA and backward discharges as ≤−28 mA, and counted over one period ( T = 142.8 μs). Experimental conditions (a): discharge electrode: Ni, winding pitch: 3 mm; (b): empty discharge zone; discharge voltage = 6 kV; discharge frequency = 7 kHz; total gas flow = 40 mL min −1 ; N 2 :H 2 = 1:1; atmospheric pressure.

Article Snippet: Thus, depending on the winding pitch, the double‐helix configuration produces 127%, 96%, and 66% higher R values than the conventional system.

Techniques: Imaging, Comparison, Concentration Assay

Journal: Chemsuschem

Article Title: Improving Plasma‐Catalytic Ammonia Synthesis Using a Coaxial Double‐Helix‐Electrode Reactor

doi: 10.1002/cssc.202502695

Figure Lengend Snippet: OES plasma parameter analysis under different discharge configurations and electrode winding pitches. (a) OES spectra of the double‐helix DBD reactor at winding pitches of 1, 3, and 5 mm; (b) OES spectrum of the conventional coaxial DBD reactor for comparison. (c) Correlation between the emission intensity ratio R = I N2+ (425 nm)/ I N2* (335 nm) and the corresponding NH 3 concentration; (d) T rot and T vib temperatures extracted by fitting the N 2 second positive system (294–384 nm) using Lifbase. Experimental conditions: Ni/Al 2 O 3 catalyst; sine waveform input; discharge voltage = 6 kV; discharge frequency = 7 kHz; total flow rate = 40 mL min −1 ; H 2 :N 2 = 1:1 (v/v); atmospheric pressure.

Article Snippet: Thus, depending on the winding pitch, the double‐helix configuration produces 127%, 96%, and 66% higher R values than the conventional system.

Techniques: Clinical Proteomics, Comparison, Concentration Assay